Comparison of the intracellular behavior of gold (Au) and indium (In) in testicle after their parenteral administration.
Identifieur interne : 000826 ( Main/Exploration ); précédent : 000825; suivant : 000827Comparison of the intracellular behavior of gold (Au) and indium (In) in testicle after their parenteral administration.
Auteurs : RBID : pubmed:23427291English descriptors
- KwdEn :
- Animals, Electron Probe Microanalysis, Gold (chemistry), Gold (metabolism), Indium (chemistry), Indium (metabolism), Leydig Cells (drug effects), Lysosomes (drug effects), Male, Microscopy, Electron, Transmission, Rats, Rats, Wistar, Sertoli Cells (drug effects), Spectrometry, Mass, Secondary Ion, Testis (drug effects).
- MESH :
- chemical , chemistry : Gold, Indium.
- chemical , metabolism : Gold, Indium.
- drug effects : Leydig Cells, Lysosomes, Sertoli Cells, Testis.
- Animals, Electron Probe Microanalysis, Male, Microscopy, Electron, Transmission, Rats, Rats, Wistar, Spectrometry, Mass, Secondary Ion.
Abstract
The subcellular behavior of several mineral elements was studied using modern techniques of observation like transmission electron microscopy and analysis like electron probe microanalysis and secondary ion mass spectrometry. In the present ultrastructural and analytical investigations, we undertake to compare the intracellular behavior of a heavy metal, gold, and a III-A group element, indium, on rat testicular tissues after their parenteral administrations. Our ultrastructural results showed that while gold was found only in the lysosomes of Leydig cells under electron dense needles, indium was observed as electron-dense deposits in the lysosomes of both Leydig and Sertoli cells. No ultrastructural modifications were observed in the testicular tissues of the control rats. The microanalytical study showed that gold was concentrated in lysosomes with sulfur as a sulfate crystalline structure whereas indium was concentrated in the same organelle as insoluble phosphate salt. These results demonstrated that testicular Leydig and Sertoli cells have the ability to selectively concentrate indium but gold was concentrated only in the first kind of cells. The mechanism implicated in this concentration phenomenon is a biochemical one involving intralysosomal hydrolytic enzymes, the acid phosphatase and the arylsulfatase. This mechanism occurs in order to protect the organism and to avoid the presence of toxic metals under soluble and free form.
DOI: 10.1093/jmicro/dft002
PubMed: 23427291
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of the intracellular behavior of gold (Au) and indium (In) in testicle after their parenteral administration.</title><author><name sortKey="Maghraoui, Samira" uniqKey="Maghraoui S">Samira Maghraoui</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Physiologie, Faculté de Médecine de Tunis, 15, Rue Djebel Lakhdar, La Rabta 1007, Tunis, Tunisie. maghraoui.samira@gmail.com</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Physiologie, Faculté de Médecine de Tunis, 15, Rue Djebel Lakhdar, La Rabta 1007, Tunis</wicri:regionArea></affiliation></author><author><name sortKey="Ayadi, Ahlem" uniqKey="Ayadi A">Ahlem Ayadi</name></author><author><name sortKey="Ben Ammar, Aouatef" uniqKey="Ben Ammar A">Aouatef Ben Ammar</name></author><author><name sortKey="Jaafoura, Mohamed Habib" uniqKey="Jaafoura M">Mohamed Habib Jaafoura</name></author><author><name sortKey="Galle, Pierre" uniqKey="Galle P">Pierre Galle</name></author><author><name sortKey="El Hili, Ali" uniqKey="El Hili A">Ali El Hili</name></author><author><name sortKey="Tekaya, Leila" uniqKey="Tekaya L">Leila Tekaya</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="RBID">pubmed:23427291</idno><idno type="pmid">23427291</idno><idno type="doi">10.1093/jmicro/dft002</idno><idno type="wicri:Area/Main/Corpus">000781</idno><idno type="wicri:Area/Main/Curation">000781</idno><idno type="wicri:Area/Main/Exploration">000826</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Electron Probe Microanalysis</term><term>Gold (chemistry)</term><term>Gold (metabolism)</term><term>Indium (chemistry)</term><term>Indium (metabolism)</term><term>Leydig Cells (drug effects)</term><term>Lysosomes (drug effects)</term><term>Male</term><term>Microscopy, Electron, Transmission</term><term>Rats</term><term>Rats, Wistar</term><term>Sertoli Cells (drug effects)</term><term>Spectrometry, Mass, Secondary Ion</term><term>Testis (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term><term>Indium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Gold</term><term>Indium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Leydig Cells</term><term>Lysosomes</term><term>Sertoli Cells</term><term>Testis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electron Probe Microanalysis</term><term>Male</term><term>Microscopy, Electron, Transmission</term><term>Rats</term><term>Rats, Wistar</term><term>Spectrometry, Mass, Secondary Ion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The subcellular behavior of several mineral elements was studied using modern techniques of observation like transmission electron microscopy and analysis like electron probe microanalysis and secondary ion mass spectrometry. In the present ultrastructural and analytical investigations, we undertake to compare the intracellular behavior of a heavy metal, gold, and a III-A group element, indium, on rat testicular tissues after their parenteral administrations. Our ultrastructural results showed that while gold was found only in the lysosomes of Leydig cells under electron dense needles, indium was observed as electron-dense deposits in the lysosomes of both Leydig and Sertoli cells. No ultrastructural modifications were observed in the testicular tissues of the control rats. The microanalytical study showed that gold was concentrated in lysosomes with sulfur as a sulfate crystalline structure whereas indium was concentrated in the same organelle as insoluble phosphate salt. These results demonstrated that testicular Leydig and Sertoli cells have the ability to selectively concentrate indium but gold was concentrated only in the first kind of cells. The mechanism implicated in this concentration phenomenon is a biochemical one involving intralysosomal hydrolytic enzymes, the acid phosphatase and the arylsulfatase. This mechanism occurs in order to protect the organism and to avoid the presence of toxic metals under soluble and free form.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23427291</PMID><DateCreated><Year>2013</Year><Month>06</Month><Day>12</Day></DateCreated><DateCompleted><Year>2014</Year><Month>01</Month><Day>08</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2050-5701</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Microscopy (Oxford, England)</Title><ISOAbbreviation>Microscopy (Oxf)</ISOAbbreviation></Journal><ArticleTitle>Comparison of the intracellular behavior of gold (Au) and indium (In) in testicle after their parenteral administration.</ArticleTitle><Pagination><MedlinePgn>397-403</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jmicro/dft002</ELocationID><Abstract><AbstractText>The subcellular behavior of several mineral elements was studied using modern techniques of observation like transmission electron microscopy and analysis like electron probe microanalysis and secondary ion mass spectrometry. In the present ultrastructural and analytical investigations, we undertake to compare the intracellular behavior of a heavy metal, gold, and a III-A group element, indium, on rat testicular tissues after their parenteral administrations. Our ultrastructural results showed that while gold was found only in the lysosomes of Leydig cells under electron dense needles, indium was observed as electron-dense deposits in the lysosomes of both Leydig and Sertoli cells. No ultrastructural modifications were observed in the testicular tissues of the control rats. The microanalytical study showed that gold was concentrated in lysosomes with sulfur as a sulfate crystalline structure whereas indium was concentrated in the same organelle as insoluble phosphate salt. These results demonstrated that testicular Leydig and Sertoli cells have the ability to selectively concentrate indium but gold was concentrated only in the first kind of cells. The mechanism implicated in this concentration phenomenon is a biochemical one involving intralysosomal hydrolytic enzymes, the acid phosphatase and the arylsulfatase. This mechanism occurs in order to protect the organism and to avoid the presence of toxic metals under soluble and free form.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Maghraoui</LastName><ForeName>Samira</ForeName><Initials>S</Initials><Affiliation>Laboratoire de Physiologie, Faculté de Médecine de Tunis, 15, Rue Djebel Lakhdar, La Rabta 1007, Tunis, Tunisie. maghraoui.samira@gmail.com</Affiliation></Author><Author ValidYN="Y"><LastName>Ayadi</LastName><ForeName>Ahlem</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ben Ammar</LastName><ForeName>Aouatef</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Jaafoura</LastName><ForeName>Mohamed Habib</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Galle</LastName><ForeName>Pierre</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>El Hili</LastName><ForeName>Ali</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Tekaya</LastName><ForeName>Leila</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>02</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microscopy (Oxf)</MedlineTA><NlmUniqueID>101595834</NlmUniqueID><ISSNLinking>2050-5698</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance>Gold</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electron Probe Microanalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gold</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Leydig Cells</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lysosomes</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Sertoli Cells</DescriptorName><QualifierName MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Spectrometry, Mass, Secondary Ion</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Testis</DescriptorName><QualifierName MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">electron probe microanalysis (EPMA)</Keyword><Keyword MajorTopicYN="N">gold</Keyword><Keyword MajorTopicYN="N">indium</Keyword><Keyword MajorTopicYN="N">lysosomes</Keyword><Keyword MajorTopicYN="N">testicular cells</Keyword><Keyword MajorTopicYN="N">transmission electron microscopy</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>2</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23427291</ArticleId><ArticleId IdType="pii">dft002</ArticleId><ArticleId IdType="doi">10.1093/jmicro/dft002</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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